More on Marketing

Saskia Ketz
2 years ago
I hate marketing for my business, but here's how I push myself to keep going
Start now.
When it comes to building my business, I’m passionate about a lot of things. I love creating user experiences that simplify branding essentials. I love creating new typefaces and color combinations to inspire logo designers. I love fixing problems to improve my product.
Business marketing isn't my thing.
This is shared by many. Many solopreneurs, like me, struggle to advertise their business and drive themselves to work on it.
Without a lot of promotion, no company will succeed. Marketing is 80% of developing a firm, and when you're starting out, it's even more. Some believe that you shouldn't build anything until you've begun marketing your idea and found enough buyers.
Marketing your business without marketing experience is difficult. There are various outlets and techniques to learn. Instead of figuring out where to start, it's easier to return to your area of expertise, whether that's writing, designing product features, or improving your site's back end. Right?
First, realize that your role as a founder is to market your firm. Being a founder focused on product, I rarely work on it.
Secondly, use these basic methods that have helped me dedicate adequate time and focus to marketing. They're all simple to apply, and they've increased my business's visibility and success.
1. Establish buckets for every task.
You've probably heard to schedule tasks you don't like. As simple as it sounds, blocking a substantial piece of my workday for marketing duties like LinkedIn or Twitter outreach, AppSumo customer support, or SEO has forced me to spend time on them.
Giving me lots of room to focus on product development has helped even more. Sure, this means scheduling time to work on product enhancements after my four-hour marketing sprint.
It also involves making space to store product inspiration and ideas throughout the day so I don't get distracted. This is like the advice to keep a notebook beside your bed to write down your insomniac ideas. I keep fonts, color palettes, and product ideas in folders on my desktop. Knowing these concepts won't be lost lets me focus on marketing in the moment. When I have limited time to work on something, I don't have to conduct the research I've been collecting, so I can get more done faster.
2. Look for various accountability systems
Accountability is essential for self-discipline. To keep focused on my marketing tasks, I've needed various streams of accountability, big and little.
Accountability groups are great for bigger things. SaaS Camp, a sales outreach coaching program, is mine. We discuss marketing duties and results every week. This motivates me to do enough each week to be proud of my accomplishments. Yet hearing what works (or doesn't) for others gives me benchmarks for my own marketing outcomes and plenty of fresh techniques to attempt.
… say, I want to DM 50 people on Twitter about my product — I get that many Q-tips and place them in one pen holder on my desk.
The best accountability group can't watch you 24/7. I use a friend's simple method that shouldn't work (but it does). When I have a lot of marketing chores, like DMing 50 Twitter users about my product, That many Q-tips go in my desk pen holder. After each task, I relocate one Q-tip to an empty pen holder. When you have a lot of minor jobs to perform, it helps to see your progress. You might use toothpicks, M&Ms, or anything else you have a lot of.
3. Continue to monitor your feedback loops
Knowing which marketing methods work best requires monitoring results. As an entrepreneur with little go-to-market expertise, every tactic I pursue is an experiment. I need to know how each trial is doing to maximize my time.
I placed Google and Facebook advertisements on hold since they took too much time and money to obtain Return. LinkedIn outreach has been invaluable to me. I feel that talking to potential consumers one-on-one is the fastest method to grasp their problem areas, figure out my messaging, and find product market fit.
Data proximity offers another benefit. Seeing positive results makes it simpler to maintain doing a work you don't like. Why every fitness program tracks progress.
Marketing's goal is to increase customers and revenues, therefore I've found it helpful to track those metrics and celebrate monthly advances. I provide these updates for extra accountability.
Finding faster feedback loops is also motivating. Marketing brings more clients and feedback, in my opinion. Product-focused founders love that feedback. Positive reviews make me proud that my product is benefitting others, while negative ones provide me with suggestions for product changes that can improve my business.
The best advice I can give a lone creator who's afraid of marketing is to just start. Start early to learn by doing and reduce marketing stress. Start early to develop habits and successes that will keep you going. The sooner you start, the sooner you'll have enough consumers to return to your favorite work.

Camilla Dudley
3 years ago
How to gain Twitter followers: A 101 Guide
No wonder brands use Twitter to reach their audience. 53% of Twitter users buy new products first.
Twitter growth does more than make your brand look popular. It helps clients trust your business. It boosts your industry standing. It shows clients, prospects, and even competitors you mean business.
How can you naturally gain Twitter followers?
Share useful information
Post visual content
Tweet consistently
Socialize
Spread your @name everywhere.
Use existing customers
Promote followers
Share useful information
Twitter users join conversations and consume material. To build your followers, make sure your material appeals to them and gives value, whether it's sales, product lessons, or current events.
Use Twitter Analytics to learn what your audience likes.
Explore popular topics by utilizing relevant keywords and hashtags. Check out this post on how to use Twitter trends.
Post visual content
97% of Twitter users focus on images, so incorporating media can help your Tweets stand out. Visuals and videos make content more engaging and memorable.
Tweet often
Your audience should expect regular content updates. Plan your ideas and tweet during crucial seasons and events with a content calendar.
Socialize
Twitter connects people. Do more than tweet. Follow industry leaders. Retweet influencers, engage with thought leaders, and reply to mentions and customers to boost engagement.
Micro-influencers can promote your brand or items. They can help you gain new audiences' trust.
Spread your @name everywhere.
Maximize brand exposure. Add a follow button on your website, link to it in your email signature and newsletters, and promote it on business cards or menus.
Use existing customers
Emails can be used to find existing Twitter clients. Upload your email contacts and follow your customers on Twitter to start a dialogue.
Promote followers
Run a followers campaign to boost your organic growth. Followers campaigns promote your account to a particular demographic, and you only pay when someone follows you.
Consider short campaigns to enhance momentum or an always-on campaign to gain new followers.
Increasing your brand's Twitter followers takes effort and experimentation, but the payback is huge.
👋 Follow me on twitter

Victoria Kurichenko
3 years ago
What Happened After I Posted an AI-Generated Post on My Website
This could cost you.
Content creators may have heard about Google's "Helpful content upgrade."
This change is another Google effort to remove low-quality, repetitive, and AI-generated content.
Why should content creators care?
Because too much content manipulates search results.
My experience includes the following.
Website admins seek high-quality guest posts from me. They send me AI-generated text after I say "yes." My readers are irrelevant. Backlinks are needed.
Companies copy high-ranking content to boost their Google rankings. Unfortunately, it's common.
What does this content offer?
Nothing.
Despite Google's updates and efforts to clean search results, webmasters create manipulative content.
As a marketer, I knew about AI-powered content generation tools. However, I've never tried them.
I use old-fashioned content creation methods to grow my website from 0 to 3,000 monthly views in one year.
Last year, I launched a niche website.
I do keyword research, analyze search intent and competitors' content, write an article, proofread it, and then optimize it.
This strategy is time-consuming.
But it yields results!
Here's proof from Google Analytics:
Proven strategies yield promising results.
To validate my assumptions and find new strategies, I run many experiments.
I tested an AI-powered content generator.
I used a tool to write this Google-optimized article about SEO for startups.
I wanted to analyze AI-generated content's Google performance.
Here are the outcomes of my test.
First, quality.
I dislike "meh" content. I expect articles to answer my questions. If not, I've wasted my time.
My essays usually include research, personal anecdotes, and what I accomplished and achieved.
AI-generated articles aren't as good because they lack individuality.
Read my AI-generated article about startup SEO to see what I mean.
It's dry and shallow, IMO.
It seems robotic.
I'd use quotes and personal experience to show how SEO for startups is different.
My article paraphrases top-ranked articles on a certain topic.
It's readable but useless. Similar articles abound online. Why read it?
AI-generated content is low-quality.
Let me show you how this content ranks on Google.
The Google Search Console report shows impressions, clicks, and average position.
Low numbers.
No one opens the 5th Google search result page to read the article. Too far!
You may say the new article will improve.
Marketing-wise, I doubt it.
This article is shorter and less comprehensive than top-ranking pages. It's unlikely to win because of this.
AI-generated content's terrible reality.
I'll compare how this content I wrote for readers and SEO performs.
Both the AI and my article are fresh, but trends are emerging.
My article's CTR and average position are higher.
I spent a week researching and producing that piece, unlike AI-generated content. My expert perspective and unique consequences make it interesting to read.
Human-made.
In summary
No content generator can duplicate a human's tone, writing style, or creativity. Artificial content is always inferior.
Not "bad," but inferior.
Demand for content production tools will rise despite Google's efforts to eradicate thin content.
Most won't spend hours producing link-building articles. Costly.
As guest and sponsored posts, artificial content will thrive.
Before accepting a new arrangement, content creators and website owners should consider this.
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Dmitrii Eliuseev
2 years ago
Creating Images on Your Local PC Using Stable Diffusion AI
Deep learning-based generative art is being researched. As usual, self-learning is better. Some models, like OpenAI's DALL-E 2, require registration and can only be used online, but others can be used locally, which is usually more enjoyable for curious users. I'll demonstrate the Stable Diffusion model's operation on a standard PC.
Let’s get started.
What It Does
Stable Diffusion uses numerous components:
A generative model trained to produce images is called a diffusion model. The model is incrementally improving the starting data, which is only random noise. The model has an image, and while it is being trained, the reversed process is being used to add noise to the image. Being able to reverse this procedure and create images from noise is where the true magic is (more details and samples can be found in the paper).
An internal compressed representation of a latent diffusion model, which may be altered to produce the desired images, is used (more details can be found in the paper). The capacity to fine-tune the generation process is essential because producing pictures at random is not very attractive (as we can see, for instance, in Generative Adversarial Networks).
A neural network model called CLIP (Contrastive Language-Image Pre-training) is used to translate natural language prompts into vector representations. This model, which was trained on 400,000,000 image-text pairs, enables the transformation of a text prompt into a latent space for the diffusion model in the scenario of stable diffusion (more details in that paper).
This figure shows all data flow:
The weights file size for Stable Diffusion model v1 is 4 GB and v2 is 5 GB, making the model quite huge. The v1 model was trained on 256x256 and 512x512 LAION-5B pictures on a 4,000 GPU cluster using over 150.000 NVIDIA A100 GPU hours. The open-source pre-trained model is helpful for us. And we will.
Install
Before utilizing the Python sources for Stable Diffusion v1 on GitHub, we must install Miniconda (assuming Git and Python are already installed):
wget https://repo.anaconda.com/miniconda/Miniconda3-py39_4.12.0-Linux-x86_64.sh
chmod +x Miniconda3-py39_4.12.0-Linux-x86_64.sh
./Miniconda3-py39_4.12.0-Linux-x86_64.sh
conda update -n base -c defaults condaInstall the source and prepare the environment:
git clone https://github.com/CompVis/stable-diffusion
cd stable-diffusion
conda env create -f environment.yaml
conda activate ldm
pip3 install transformers --upgradeDownload the pre-trained model weights next. HiggingFace has the newest checkpoint sd-v14.ckpt (a download is free but registration is required). Put the file in the project folder and have fun:
python3 scripts/txt2img.py --prompt "hello world" --plms --ckpt sd-v1-4.ckpt --skip_grid --n_samples 1Almost. The installation is complete for happy users of current GPUs with 12 GB or more VRAM. RuntimeError: CUDA out of memory will occur otherwise. Two solutions exist.
Running the optimized version
Try optimizing first. After cloning the repository and enabling the environment (as previously), we can run the command:
python3 optimizedSD/optimized_txt2img.py --prompt "hello world" --ckpt sd-v1-4.ckpt --skip_grid --n_samples 1Stable Diffusion worked on my visual card with 8 GB RAM (alas, I did not behave well enough to get NVIDIA A100 for Christmas, so 8 GB GPU is the maximum I have;).
Running Stable Diffusion without GPU
If the GPU does not have enough RAM or is not CUDA-compatible, running the code on a CPU will be 20x slower but better than nothing. This unauthorized CPU-only branch from GitHub is easiest to obtain. We may easily edit the source code to use the latest version. It's strange that a pull request for that was made six months ago and still hasn't been approved, as the changes are simple. Readers can finish in 5 minutes:
Replace if attr.device!= torch.device(cuda) with if attr.device!= torch.device(cuda) and torch.cuda.is available at line 20 of ldm/models/diffusion/ddim.py ().
Replace if attr.device!= torch.device(cuda) with if attr.device!= torch.device(cuda) and torch.cuda.is available in line 20 of ldm/models/diffusion/plms.py ().
Replace device=cuda in lines 38, 55, 83, and 142 of ldm/modules/encoders/modules.py with device=cuda if torch.cuda.is available(), otherwise cpu.
Replace model.cuda() in scripts/txt2img.py line 28 and scripts/img2img.py line 43 with if torch.cuda.is available(): model.cuda ().
Run the script again.
Testing
Test the model. Text-to-image is the first choice. Test the command line example again:
python3 scripts/txt2img.py --prompt "hello world" --plms --ckpt sd-v1-4.ckpt --skip_grid --n_samples 1The slow generation takes 10 seconds on a GPU and 10 minutes on a CPU. Final image:
Hello world is dull and abstract. Try a brush-wielding hamster. Why? Because we can, and it's not as insane as Napoleon's cat. Another image:
Generating an image from a text prompt and another image is interesting. I made this picture in two minutes using the image editor (sorry, drawing wasn't my strong suit):
I can create an image from this drawing:
python3 scripts/img2img.py --prompt "A bird is sitting on a tree branch" --ckpt sd-v1-4.ckpt --init-img bird.png --strength 0.8It was far better than my initial drawing:
I hope readers understand and experiment.
Stable Diffusion UI
Developers love the command line, but regular users may struggle. Stable Diffusion UI projects simplify image generation and installation. Simple usage:
Unpack the ZIP after downloading it from https://github.com/cmdr2/stable-diffusion-ui/releases. Linux and Windows are compatible with Stable Diffusion UI (sorry for Mac users, but those machines are not well-suitable for heavy machine learning tasks anyway;).
Start the script.
Done. The web browser UI makes configuring various Stable Diffusion features (upscaling, filtering, etc.) easy:
V2.1 of Stable Diffusion
I noticed the notification about releasing version 2.1 while writing this essay, and it was intriguing to test it. First, compare version 2 to version 1:
alternative text encoding. The Contrastive LanguageImage Pre-training (CLIP) deep learning model, which was trained on a significant number of text-image pairs, is used in Stable Diffusion 1. The open-source CLIP implementation used in Stable Diffusion 2 is called OpenCLIP. It is difficult to determine whether there have been any technical advancements or if legal concerns were the main focus. However, because the training datasets for the two text encoders were different, the output results from V1 and V2 will differ for the identical text prompts.
a new depth model that may be used to the output of image-to-image generation.
a revolutionary upscaling technique that can quadruple the resolution of an image.
Generally higher resolution Stable Diffusion 2 has the ability to produce both 512x512 and 768x768 pictures.
The Hugging Face website offers a free online demo of Stable Diffusion 2.1 for code testing. The process is the same as for version 1.4. Download a fresh version and activate the environment:
conda deactivate
conda env remove -n ldm # Use this if version 1 was previously installed
git clone https://github.com/Stability-AI/stablediffusion
cd stablediffusion
conda env create -f environment.yaml
conda activate ldmHugging Face offers a new weights ckpt file.
The Out of memory error prevented me from running this version on my 8 GB GPU. Version 2.1 fails on CPUs with the slow conv2d cpu not implemented for Half error (according to this GitHub issue, the CPU support for this algorithm and data type will not be added). The model can be modified from half to full precision (float16 instead of float32), however it doesn't make sense since v1 runs up to 10 minutes on the CPU and v2.1 should be much slower. The online demo results are visible. The same hamster painting with a brush prompt yielded this result:
It looks different from v1, but it functions and has a higher resolution.
The superresolution.py script can run the 4x Stable Diffusion upscaler locally (the x4-upscaler-ema.ckpt weights file should be in the same folder):
python3 scripts/gradio/superresolution.py configs/stable-diffusion/x4-upscaling.yaml x4-upscaler-ema.ckptThis code allows the web browser UI to select the image to upscale:
The copy-paste strategy may explain why the upscaler needs a text prompt (and the Hugging Face code snippet does not have any text input as well). I got a GPU out of memory error again, although CUDA can be disabled like v1. However, processing an image for more than two hours is unlikely:
Stable Diffusion Limitations
When we use the model, it's fun to see what it can and can't do. Generative models produce abstract visuals but not photorealistic ones. This fundamentally limits The generative neural network was trained on text and image pairs, but humans have a lot of background knowledge about the world. The neural network model knows nothing. If someone asks me to draw a Chinese text, I can draw something that looks like Chinese but is actually gibberish because I never learnt it. Generative AI does too! Humans can learn new languages, but the Stable Diffusion AI model includes only language and image decoder brain components. For instance, the Stable Diffusion model will pull NO WAR banner-bearers like this:
V1:
V2.1:
The shot shows text, although the model never learned to read or write. The model's string tokenizer automatically converts letters to lowercase before generating the image, so typing NO WAR banner or no war banner is the same.
I can also ask the model to draw a gorgeous woman:
V1:
V2.1:
The first image is gorgeous but physically incorrect. A second one is better, although it has an Uncanny valley feel. BTW, v2 has a lifehack to add a negative prompt and define what we don't want on the image. Readers might try adding horrible anatomy to the gorgeous woman request.
If we ask for a cartoon attractive woman, the results are nice, but accuracy doesn't matter:
V1:
V2.1:
Another example: I ordered a model to sketch a mouse, which looks beautiful but has too many legs, ears, and fingers:
V1:
V2.1: improved but not perfect.
V1 produces a fun cartoon flying mouse if I want something more abstract:
I tried multiple times with V2.1 but only received this:
The image is OK, but the first version is closer to the request.
Stable Diffusion struggles to draw letters, fingers, etc. However, abstract images yield interesting outcomes. A rural landscape with a modern metropolis in the background turned out well:
V1:
V2.1:
Generative models help make paintings too (at least, abstract ones). I searched Google Image Search for modern art painting to see works by real artists, and this was the first image:
I typed "abstract oil painting of people dancing" and got this:
V1:
V2.1:
It's a different style, but I don't think the AI-generated graphics are worse than the human-drawn ones.
The AI model cannot think like humans. It thinks nothing. A stable diffusion model is a billion-parameter matrix trained on millions of text-image pairs. I input "robot is creating a picture with a pen" to create an image for this post. Humans understand requests immediately. I tried Stable Diffusion multiple times and got this:
This great artwork has a pen, robot, and sketch, however it was not asked. Maybe it was because the tokenizer deleted is and a words from a statement, but I tried other requests such robot painting picture with pen without success. It's harder to prompt a model than a person.
I hope Stable Diffusion's general effects are evident. Despite its limitations, it can produce beautiful photographs in some settings. Readers who want to use Stable Diffusion results should be warned. Source code examination demonstrates that Stable Diffusion images feature a concealed watermark (text StableDiffusionV1 and SDV2) encoded using the invisible-watermark Python package. It's not a secret, because the official Stable Diffusion repository's test watermark.py file contains a decoding snippet. The put watermark line in the txt2img.py source code can be removed if desired. I didn't discover this watermark on photographs made by the online Hugging Face demo. Maybe I did something incorrectly (but maybe they are just not using the txt2img script on their backend at all).
Conclusion
The Stable Diffusion model was fascinating. As I mentioned before, trying something yourself is always better than taking someone else's word, so I encourage readers to do the same (including this article as well;).
Is Generative AI a game-changer? My humble experience tells me:
I think that place has a lot of potential. For designers and artists, generative AI can be a truly useful and innovative tool. Unfortunately, it can also pose a threat to some of them since if users can enter a text field to obtain a picture or a website logo in a matter of clicks, why would they pay more to a different party? Is it possible right now? unquestionably not yet. Images still have a very poor quality and are erroneous in minute details. And after viewing the image of the stunning woman above, models and fashion photographers may also unwind because it is highly unlikely that AI will replace them in the upcoming years.
Today, generative AI is still in its infancy. Even 768x768 images are considered to be of a high resolution when using neural networks, which are computationally highly expensive. There isn't an AI model that can generate high-resolution photographs natively without upscaling or other methods, at least not as of the time this article was written, but it will happen eventually.
It is still a challenge to accurately represent knowledge in neural networks (information like how many legs a cat has or the year Napoleon was born). Consequently, AI models struggle to create photorealistic photos, at least where little details are important (on the other side, when I searched Google for modern art paintings, the results are often even worse;).
When compared to the carefully chosen images from official web pages or YouTube reviews, the average output quality of a Stable Diffusion generation process is actually less attractive because to its high degree of randomness. When using the same technique on their own, consumers will theoretically only view those images as 1% of the results.
Anyway, it's exciting to witness this area's advancement, especially because the project is open source. Google's Imagen and DALL-E 2 can also produce remarkable findings. It will be interesting to see how they progress.

Woo
3 years ago
How To Launch A Business Without Any Risk
> Say Hello To The Lean-Hedge Model
People think starting a business requires significant debt and investment. Like Shark Tank, you need a world-changing idea. I'm not saying to avoid investors or brilliant ideas.
Investing is essential to build a genuinely profitable company. Think Apple or Starbucks.
Entrepreneurship is risky because many people go bankrupt from debt. As starters, we shouldn't do it. Instead, use lean-hedge.
Simply defined, you construct a cash-flow business to hedge against long-term investment-heavy business expenses.
What the “fx!$rench-toast” is the lean-hedge model?
When you start a business, your money should move down, down, down, then up when it becomes profitable.
Many people don't survive the business's initial losses and debt. What if, we created a cash-flow business BEFORE we started our Starbucks to hedge against its initial expenses?
Lean-hedge has two sections. Start a cash-flow business. A cash-flow business takes minimal investment and usually involves sweat and time.
Let’s take a look at some examples:
A Translation company
Personal portfolio website (you make a site then you do cold e-mail marketing)
FREELANCE (UpWork, Fiverr).
Educational business.
Infomarketing. (You design a knowledge-based product. You sell the info).
Online fitness/diet/health coaching ($50-$300/month, calls, training plan)
Amazon e-book publishing. (Medium writers do this)
YouTube, cash-flow channel
A web development agency (I'm a dev, but if you're not, a graphic design agency, etc.) (Sell your time.)
Digital Marketing
Online paralegal (A million lawyers work in the U.S).
Some dropshipping (Organic Tik Tok dropshipping, where you create content to drive traffic to your shopify store instead of spend money on ads).
(Disclaimer: My first two cash-flow enterprises, which were language teaching, failed terribly. My translation firm is now booming because B2B e-mail marketing is easy.)
Crossover occurs. Your long-term business starts earning more money than your cash flow business.
My cash-flow business (freelancing, translation) makes $7k+/month.
I’ve decided to start a slightly more investment-heavy digital marketing agency
Here are the anticipated business's time- and money-intensive investments:
($$$) Top Front-End designer's Figma/UI-UX design (in negotiation)
(Time): A little copywriting (I will do this myself)
($$) Creating an animated webpage with HTML (in negotiation)
Backend Development (Duration) (I'll carry out this myself using Laravel.)
Logo Design ($$)
Logo Intro Video for $
Video Intro (I’ll edit this myself with Premiere Pro)
etc.
Then evaluate product, place, price, and promotion. Consider promotion and pricing.
The lean-hedge model's point is:
Don't gamble. Avoid debt. First create a cash-flow project, then grow it steadily.
Check read my previous posts on “Nightmare Mode” (which teaches you how to make work as interesting as video games) and Why most people can't escape a 9-5 to learn how to develop a cash-flow business.

Bob Service
3 years ago
Did volcanic 'glasses' play a role in igniting early life?
Quenched lava may have aided in the formation of long RNA strands required by primitive life.
It took a long time for life to emerge. Microbes were present 3.7 billion years ago, just a few hundred million years after the 4.5-billion-year-old Earth had cooled enough to sustain biochemistry, according to fossils, and many scientists believe RNA was the genetic material for these first species. RNA, while not as complicated as DNA, would be difficult to forge into the lengthy strands required to transmit genetic information, raising the question of how it may have originated spontaneously.
Researchers may now have a solution. They demonstrate how basaltic glasses assist individual RNA letters, also known as nucleoside triphosphates, join into strands up to 200 letters long in lab studies. The glasses are formed when lava is quenched in air or water, or when melted rock generated by asteroid strikes cools rapidly, and they would have been plentiful in the early Earth's fire and brimstone.
The outcome has caused a schism among top origin-of-life scholars. "This appears to be a great story that finally explains how nucleoside triphosphates react with each other to create RNA strands," says Thomas Carell, a scientist at Munich's Ludwig Maximilians University. However, Harvard University's Jack Szostak, an RNA expert, says he won't believe the results until the study team thoroughly describes the RNA strands.
Researchers interested in the origins of life like the idea of a primordial "RNA universe" since the molecule can perform two different functions that are essential for life. It's made up of four chemical letters, just like DNA, and can carry genetic information. RNA, like proteins, can catalyze chemical reactions that are necessary for life.
However, RNA can cause headaches. No one has yet discovered a set of plausible primordial conditions that would cause hundreds of RNA letters—each of which is a complicated molecule—to join together into strands long enough to support the intricate chemistry required to kick-start evolution.
Basaltic glasses may have played a role, according to Stephen Mojzsis, a geologist at the University of Colorado, Boulder. They're high in metals like magnesium and iron, which help to trigger a variety of chemical reactions. "Basaltic glass was omnipresent on Earth at the time," he adds.
He provided the Foundation for Applied Molecular Evolution samples of five different basalt glasses. Each sample was ground into a fine powder, sanitized, and combined with a solution of nucleoside triphosphates by molecular biologist Elisa Biondi and her colleagues. The RNA letters were unable to link up without the presence of glass powder. However, when the molecules were mixed with the glass particles, they formed long strands of hundreds of letters, according to the researchers, who published their findings in Astrobiology this week. There was no need for heat or light. Biondi explains, "All we had to do was wait." After only a day, little RNA strands produced, yet the strands continued to grow for months. Jan Paek, a molecular biologist at Firebird Biomolecular Sciences, says, "The beauty of this approach is its simplicity." "Mix the components together, wait a few days, and look for RNA."
Nonetheless, the findings pose a slew of problems. One of the questions is how nucleoside triphosphates came to be in the first place. Recent study by Biondi's colleague Steven Benner suggests that the same basaltic glasses may have aided in the creation and stabilization of individual RNA letters.
The form of the lengthy RNA strands, according to Szostak, is a significant challenge. Enzymes in modern cells ensure that most RNAs form long linear chains. RNA letters, on the other hand, can bind in complicated branching sequences. Szostak wants the researchers to reveal what kind of RNA was produced by the basaltic glasses. "It irritates me that the authors made an intriguing initial finding but then chose to follow the hype rather than the research," Szostak says.
Biondi acknowledges that her team's experiment almost probably results in some RNA branching. She does acknowledge, however, that some branched RNAs are seen in species today, and that analogous structures may have existed before the origin of life. Other studies carried out by the study also confirmed the presence of lengthy strands with connections, indicating that they are most likely linear. "It's a healthy argument," says Dieter Braun, a Ludwig Maximilian University origin-of-life chemist. "It will set off the next series of tests."
